Fluorochromes

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Common Fluorochromes for the
FACSCalibur and FACSAria III

 spectrum

FACSCalibur:

488 nm Laser

FL1 - 530/30
Fluorescein (FITC)
CFSE
Alexafluor 488
eGFP

FL2 - 585/42
Phycoerythrin (PE)
Propidium Iodide (PI)

FL3 - 670 LP
PerCP
PerCP-Cy5.5
PE-Cy5
PE-Cy7
Propidium Iodide (PI)
7-AAD

635 nm Laser

FL4 - 661/16
Allophycocyanin (APC)
Cy5
Alexa Fluor 633
Alexafluor 647

 

 

 

 

 

 

FACSAria III:

488 nm Laser

Detector E - 530/30
Fluorescein (FITC)
CFSE
Alexafluor 488
eGFP

Detector D - 585/42
Phycoerythrin (PE)

Detector C - 616/23
PE-Texas Red
Propidium Iodide (PI)
PE-Alexa Fluor 610

Detector B - 695/40
PE-Cy5
PerCP-Cy5.5
7-AAD
PE-Cy5.5

Detector A - 780/60
PE-Cy7
PE-Alexa Fluor 750

633 nm Laser

Detector B - 660/20
Allophycocyanin (APC)
Alexa Fluor 633
Alexa Fluor 647
Cy5

Detector A - 780/60
APC-Cy7
APC-H7
APC-Alexa Fluor 750
APC-eFluor 780