Stephen B. Pruett, B.S., Ph.D.
Department of Basic Sciences
College of Veterinary Medicine
PO Box 6100
Mississippi State, MS
Phone: (662) 325-6653
Fax: (662) 325-8884 or (662) 325-1031
- B. S. – Northwestern State University
- Ph.D. – LSU School of Medicine in Shreveport
- Mechanisms of immunomodulation by drugs and chemicals with particular emphasis on the role of neuroendocrine mediators. Mathimatical/statistical modeling of immune function.
- Immunology and Toxicology
Rodent models of infectious diseases and cancer with emphasis on the immune system and toxicology; flow cytometry; luminescent imaging using mice with reporter genes (NF-kappaB); microarray analysis (transcriptomics); Ingenuity pathway analysis; immunological assays such as commercial or customized ELISA, multiplexed bead array (Luminex), NK cell assay, total and specific antibodies, differential cell counts; general assays for, apoptosis; cellular viability; cell cycle analysis; cellular signaling (by western blot and using ELISA-like kits); transcription factor activation/inhibition. Circular dichroism to evaluate conformational change in purified proteins (in collaboration with Ed Lewis in the Department of Chemistry); mouse model for binge drinking of alcohol; mouse and rat models of stress effects, including quantitative assessment using area under the corticosterone concentration vs. time curve; distinguishing stress effects from other mechanisms of action of immunotoxicants; mathematical modeling of host resistance to infection (in collaboration with Changhe Yuan, Computer Science and Engineering); mechanisms of pathogenicity of Streptococcus pneumoniae and mechanisms of lethality in sepsis and effects of ethanol on both of these (in collaboration with Bindu Nanduri). Most of these techniques could be available for a collaborative study, but the limited number of personnel in my lab cannot support more than one or two additional collaborations or students.