Faculty Bio
C.P. Coyne, DVM., PhD., DACVIM
Professor, Molecular Pharmacology and Immunology
Department of Basic Sciences
Contact Information
College of Veterinary Medicine
P.O. Box 6100
Mississippi State, MS 39762-6100
Phone: 662-325-1120
Email: coyne@cvm.msstate.edu
Education
- D.V.M. – Colorado State University
- Internship – University of California-Davis
- Ph.D. – University of California at Davis
- Residency – University of California at Davis
- Diplomate – American College of Veterinary Internal Medicine
Special Interests
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Research – Application of molecular biology and proteomics analyses to delineate mechanisms of disease associated with immunological processes and neoplastic conditions as an investigational basis for the molecular design of pharmaceutical and biopharmaceutical agents. Investigations have also been devoted to the development of novel vaccine preparations.
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Teaching – Graduate Research Students: pharmaceutical molecular design, molecular oncology, proteomics, modification of gene transcription, molecular pathogenesis of septic and non-septic inflammatory reactions Veterinary Students: pharmacology and internal medicine
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Research - Molecular Pharmacology
Primary Area of Expertise
- Discovery of molecular mechanisms of disease (biochemical / genetic) "targets" that can be applied as templates for the design of pharmaceutical and biopharmaceutical agents.
- Anti-neoplatic properties of electromagnetic fields
- Strategies for modifying the molecular pathogenesis of intense septic and non-septic inflammatory responses
- Strategies for modifying the molecular pathogenesis of hyper-immune and auto-immune disease states
- Mononuclear cell (macrophage/lymphocyte) and neoplastic cell proteomics
- Diagnotic pharmacology in investigative research and clinical medicine
Secondary Area of Expertise
Parasite vaccine development
Publications
Publication of Research Manuscripts and Contributions to the Medical Literature
Coyne CP (MSU), Jones T, Bear R. Synthesis of gemcitabine-(C4-amide)-[anti-HER2/neu] utilizing a UV-photoactivated gemcitabine intermediate: cytotoxic anti-Neoplastic activity against chemotherapeutic-resistant mammary adenocarcinoma SKBr-3. Accepted August 14, 2012 Journal of Cancer Therapy: Breast Cancer Special Issue.
Coyne CP (MSU), Jones T, Bear R. Influence of alternative tubulin inhibitors on the potency of epirubicin-SS-anti-HER2/neu synthesized with a UV-activated intermediate. Journal of Cancer and Clinical Oncology 2012: 1(2) (page numbers pending) ISSN 1927-4858 E-ISSN 1927-4866
Coyne CP (MSU), Jones T, Bear R. Synthesis of a covalent epirubicin-(C3-amide)-anti-HER2/neu immunochemotherapeutic utilizing a UV-photoactivated anthracycline intermediate. Coyne CP, Jones T, Bear R. Cancer Biotherapy and Radiopharmaceuticals 2012:27(1) 41-55.
Coyne CP (MSU), Jones T, Sygula A, Bailey J, Pinchuk L. Epirubicin-[anti-HER2/neu] synthesized with an epirubicin-(C13-keto) EMCS intermediate: anti-neoplastic activity against chemotherapeutic-resistant SKBr-3 mammary adenocarcinoma in combination with organic selenium. Journal of Cancer Therapy. 2011;2(1):22-39.
Coyne CP (MSU), Jones T, Pharr T. Synthesis of a covalent gemcitabine-(carbamate)-[anti-HER2/neu] immunochemotherapeutic and cytotoxic anti-neoplastic activity against chemotherapeutic-resistant SKBr-3 mammary adenocarcinoma. Bioorganic and Medicinal Chemistry 2011;19:67-76.
Coyne CP (MSU), Ross MK, Bailey JG. Dual potency anti-HER2/neu and anti-EGFR anthracycline immunoconjugates in chemotherapeutic-resistant mammary adenocarcinoma combined with cyclosporin A and verapamil P-glycoprotein inhibition. Journal of Drug Targeting 2009;17(6):474-489.
Coyne CP (MSU), Rashmir-Raven A, Jones T, Mochal C, Linford RL, Brashier M, Eddy A. Delineation of pulmonary airway fluid protein fractions with HRPO binding-avidity by far-Western ligand blot and mass spectrometry analyses: A model methodology for detecting mannose-binding protein expression profiles. ACTA BioMedica 2009;80:243-261.
Coyne CP (MSU), Cadillac JM. SPECIAL REPORT Factors Associated with Amounts of Research Funding Obtained from the National Institutes of Health by Colleges and Schools of Veterinary Medicine. Am J Vet Res 2008;69(12):1676-1685 (Reviewed: AVMA Research Board and CVM Deans, AJVR Editorial Board)
Coyne CP (MSU), Howell T, Baravik J, Baravik E, Fenwick BW, Willetto C. Biochemical mechanisms influencing the expression of membrane-associated TNF RII (75-kDa) and IL-1 RI (80 kDa) receptor complexes in adherent populations of vascular endothelium. Pathophys 2003;9:115-125.
Coyne CP (MSU), Howell T, Smodlaka H, Willetto C, Fenwick BW, Chenney E. Biochemical mechanisms that promote variable expression and generate soluble fragments of membrane-associated CD14 complexes in adherent macrophages. Journal of Endotoxin Res 2002;8:273-283.
Coyne CP (MSU), Baravik J, Baravik E, Howell T, Fenwick BW, Willetto C. Biochemical alteration of membrane-associated IL-6 RI (80-kDa) shedding in populations of adherent macrophages and vascular endothelium. Molecular Immunology 2001;38:347-357.
Coyne CP (MSU), Baravik J, Howell T, Baravik E, Fenwick BW, Willetto C. Biochemical mechanisms that interact with membrane-associated IL-1 RII (60-kDa decoy) receptors in populations of adherent macrophages and vascular endothelium. Cellular Signaling 2001;12;765-776.
Note: the four research manuscripts listed immediately above represent the first investigations to delineate the biochemical mechanisms involved in the protective cytokine receptor “shedding” phenomenon.
Coyne CP (MSU), Brake D. Characterization of Haemonchus contortus-derived cell populations propagated in-vitro in a tissue culture environment and their potential to induce protective immunity in sheep. International Journal of Parasitol 2001;31:359-376.
Coyne CP (MSU), Willetto C, Fenwick BW. A mechanism of TNFR Type II (75-kDa) “shedding” in macrophages. Shock 1999;1:19-30.
Pond AL, Chambers HW, Coyne CP (MSU), Chambers JE. Purification of two rat hepatic proteins with A-esterase activity. J Pharm Exp Therp 1998;286:1404-1411.
Coyne CP (MSU), Fenwick BW, Ainsworth J. Anti-neoplastic activity of chemotherapeutic "loaded" neutrophils against human mammary adenocarcinoma. Biotherapy 1997;10:145-159.
Pond AL, Coyne CP (MSU), Chamber HW, Chambers JE. Identification and isolation of two rat serum proteins with A-esterase activity towards paraoxon and chlorpyrifos-oxon. Biochem Pharm 1996;52:363-369.
Coyne CP (MSU), Moritz JT, Fenwick BW. Inhibition of lipopolysaccharide-induced TNF-alpha production by semisynthetic polymyxin-B conjugated dextran. Biotechnology Therapeutics 1994-1995, 5(3-4) 137-162. (MSU: Application Submitted to United States Patent Office).
Coyne CP (MSU), Moritz J. Semi-synthesis of polymyxin-B conjugated ovalbumin: evaluation of lipopolysaccharide binding-avidity and neutralization of induced TNF-a synthesis. Biotherapy 1995;8:69-83 (MSU: Application Submitted to United States Patent Office).
Coyne CP (MSU), Moritz J. Design of a semi-synthetic (polymyxin-B•biotin)4|avidin tetramer and evaluation of it's ability to inhibit lipopolysaccharide induced TNF-a synthesis. J Endotox Res 1994;207-215. (MSU: Application Submitted to United States Patent Office).
Coyne CP (KSU), Fenwick BW, Iandola J, Griffith G, Williams D. Cytoprotective properties of an equine TNF-a monokine inhibitor liberated by isolated white blood cell populations following chemotactant stimulation. Am J Vet Res 1993;54:845-855.
Coyne CP (KSU), Fenwick BW. Inhibition of lipopolysaccharide-induced macrophage TNF-a synthesis by polymyxin-B. Am J Vet Res 1993;54:305-314.
Erickson HH, Olsen S, Pelletier N, Lowe B, Coyne CP (KSU). Exercise induced pulmonary hemorrhage in equine athletes. Eq Nutr Physiol Proc 1993;13:132-133. (co-major professor for PhD graduate students).
Erickson HH, Olsen S, Pelletier N, Lowe B, Coyne CP (KSU). Reversal of furosemide induced hemodynamic alteration by NSAIDS. Am Assoc Eq Pract. 1993;735-738. (co-major professor for PhD graduate students)
Coyne CP (KSU), Smith JE, Keeton K. Additive and synergistic pharmacological inhibition of factor XIIIa*-like biochemical activity. Am J Vet Res 1992;53:2058-2066.
Coyne CP (KSU), Smith JE, DeBowes RM. Pharmacologic evaluation of factor XIIIa*-like enzyme activity in equine plasma as a potential therapeutic avenue for the inhibition of fibrinous tissue. Am J Vet Res 1992;53:695-705.
Rashmir A, Coyne CP (KSU), Fenwick B, DeBowes R. Inhibition of complement by therapeutic polysulfated glycosaminoglycans (Adequan). Am J Vet Res 1992;53:87-90.
Olsen SC, Coyne CP (KSU), Lowe BS,* Pelletier N,* Raub EM, Erickson HH. Influence of cyclooxygenase inhibitors on furosemide-induced hemodynamic effects during exercise in horses. Am J Vet Res 1992;53:1562-1567.
Note: Manuscript listed on the Maxwell Gluck Equine Research Center Website as a Selected Reference for Exercise-Induced Pulmonary Hemmorhage in Horses (1990-2001)
Olsen SC, Coyne CP (KSU), Lowe BS, Pelletier N, Raub EM, Erickson HH. Influence of furosemide on hemodynamic responses during exercise in the horse. Am J Vet Res 1992;53:742-747.
Note: Manuscript listed on the Maxwell Gluck Equine Research Center Website as a Selected Reference for Exercise-Induced Pulmonary Hemmorhage in Horses (1990-2001)
Erickson HH, Hopper MK, Olsen SC, Pelletier NG, Lowe BS, Coyne CP (KSU). Cardiopulmonary mechanisms of exercise-induced pulmonary hemorrhage (EIPH) and action of furosemide. AAEP Proc 1991;37:651-661.
Note: Manuscript listed on the Maxwell Gluck Equine Research Center Website as a Selected Reference for Exercise-Induced Pulmonary Hemmorhage in Horses (1990-2001)
Erickson HH, Erickson MK, Hopper MK, Olsen SC, Pelletier N, Lowe BS, Coyne CP (KSU). Cardiopulmonary mechanism of exercise-induced pulmonary hemorrhage (EIPH) and furosemide. The Quarter Horse J. 1991;43(8):110.
Note: Manuscript listed on the Maxwell Gluck Equine Research Center Website as a Selected Reference for Exercise-Induced Pulmonary Hemmorhage in Horses (1990-2001)
Coyne CP (UC-Davis), Carlson GP, Spensley M, Case J. Preliminary investigation of alterations in blood viscosity, cellular composition, and electrophoresis plasma protein faction profile after competitive racing activity in Thoroughbred horses. Am J Vet Res 1990;51:1956-1963.
Coyne CP (UC-Davis), Kelly AB, Hornof WJ, O'Brien TR, Phelps MS, Lamb JF. Radiolabeling of equine platelets in plasma with [111In]-2_mercaptopyridine_N_oxide and their in-vivo survival in the horse. Am J Vet Res 1987;48:385-391.
Coyne CP (UC-Davis), Hornof WJ, Kelly AB, O'Brien TR, DeNardo SJ. Extraction, radio-iodination, and in-vivo catabolism of equine fibrinogen. Am J Vet Res 1985;46:2572_2577.
Coyne CP (UC-Davis), Hornof WJ, Kelly AB, O'Brien TR, DeNardo SJ. Rapid extraction, radioiodination, and in-vivo catabolism of [125I]_labeled fibrinogen in the horse. Am J Vet Res 1985:46:2578_2581.
Electronic Veterinary Clinical Reference Database Authorship Contributions
- VetStream Reviewer (April 2002 to May 2003)
- Three Hills Farm, Bartlow Cambridge United Kingdom.
Author and Editorial Review for International Veterinary Database (CD Format)
- Vetstream Felis: Immunology (quarterly contributions)
- Vetstream Equis: Immunology (quarterly contributions)
- Vetstream Equis: Oncology (quarterly contributions)
Published Veterinary Medical Manuscripts (Peer Reviewed)
Hodder A, Coyne CP (MSU), Madigan J. Birth of live triplets in a mare. Case Report. Equine Veterinary Journal 2010;42(1):84-85.
Whitehair KJ,r Cox JH, Coyne CP (KSU), DeBowes RM. Esophageal obstruction in horses. Comp Cont Ed 1990;12(1):91-96.
Coyne CP (CSU). Equine Protozoal Encephalomyelitis In. Assorted Topics in Equine Lameness. Turner AS. eds. Colorado State University Press. 1981;285-299.
Published Manuscripts Reporting Veterinary Clinical Research (Peer Reviewed)
Koblik P, O'Brien TR, Coyne CP (KSU). Effect of radiographic tomography positioning on measurement of distal phalangeal rotational angle in horses with laminitis. J Am Vet Med Assoc 192(3):346_349;1988.
Textbooks Related to Research Related to the Medical Health Professions (Peer Reviewed)
Comparative Diagnostic Pharmacology: Clinical and Research Applications in Living-System Models. C.P. Coyne (editor/author) Wiley-Blackwell Publishing LTD, Oxford United Kingdom, 2006, Chap 1-22, pp. 1-965.
Publications in Veterinary Clinical Textbooks (Invited Contributions)
Coyne CP (KSU), Oehme F. Natural Toxins In. Large Animal Internal Medicine. Smith BP, ed. Mosby, St. Louis, 1990;1646-1668.
Coyne CP (KSU). Equine Neurologic Examination. In. Current Therapy in Equine Internal Medicine III. Robinson NE. eds. W.B. Saunders, Philadelphia, 1991;521-523.
Coyne CP (KSU). Equine Cerebrospinal Fluid Collection and Analysis. In. Current Therapy in Equine Internal Medicine III. Robinson NE. eds. W.B. Saunders, Philadelphia, 1991;523-525.
Liu D, Pruett SB, Coyne CP. Alternaria, In Molecular Detection of Foodborne Pathogens. Dongyou Liu D. ed. CRC Press, 2009; Chap 37, 513-518.
